_mechanics-and-single-molecule-interrogation-of-dna-recombination-supplemental-video-7.jpg "Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 7 from www reca Video Screenshot Preview")
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⏲ Duration: 44 sec
✓ Published: 12-Apr-2016
✓ Published: 12-Apr-2016
Description:
Supplemental Video 7: Direct imaging of nucleation and growth of RecA on SSB-coated ssDNA using TIRF microscopy. nThis video first shows a flow-extended single molecule of 3′-biotinylated λ ssDNA coated with a fluorescent protein, SSBAF488. The molecule was tethered to a streptavidin-coated glass surface within a microfluidic flow cell and visualized using total internal reflection fluorescence microscopy. Solution flow is left to right. SSBAF488 was exchanged with non-fluorescent SSB on the
Supplemental Video 7: Direct imaging of nucleation and growth of RecA on SSB-coated ssDNA using TIRF microscopy. nThis video first shows a flow-extended single molecule of 3′-biotinylated λ ssDNA coated with a fluorescent protein, SSBAF488. The molecule was tethered to a streptavidin-coated glass surface within a microfluidic flow cell and visualized using total internal reflection fluorescence microscopy. Solution flow is left to right. SSBAF488 was exchanged with non-fluorescent SSB on the
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